Peran Lipopolisakarida Helicobacter pylori terhadap Aktivitas Neutrofil pada Penderita Infark Miokard Akut melalui Degradasi Kolagen Tipe IV
Abstract
Matrix metalloproteases (MMPs) are proteolytic enzymes that play a criti-cal role in decreasing the stability of atherosclerotic plaque causing Acute Myocardial Infarction (AMI). In this process, degradation of type IV col-lagen as an important component of endothelial membrane is thought to be important. Several microorganisms and their products including Li-popolysaccharide (LPS) have been suggested to be able to activate MMPs.The aim of this study is to investigate the role of Helicobacter pyloriLPS in type IV collagen degradation (in vitro). We used two stages in this study: MMP production stage and degradation of type IV collagen. MMP derived from the neutrophils of AMI and non-AMI patients that had been stimulated with LPS of Helicobacter pyloriwere used to digest type IV collagen. Analysis of type IV collagen degradation was performed with SDS-PAGE and Western blot.
This study showed MMP isolated from neutrophil of AMI patients is an active MMP-9 at Molecular weight of 72 kDa. It can degrade type IV col-lagen at MW 78.6 kDa, 60 kDa, 50 kDa, 47.8 kDa and 43.8 kDa. Whereas MMP isolated from neutrophil of non-AMI have MW of 91.2 kDa and can only degrade collagen IVat merely 60 kDa.
In conclusion, Helicobacter pyloriLPS is able to induce neutrophil of AMI patients to produce an active MMP-9 at MW 72 kDa and the enzyme may play strategic role in the degradation of type IV collagen.
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Ameriso, S.F.; A.F. Esteban; C.L. Ramon; E.S. Gustavo, 2001. Detection of Helicobacter pyloriin Human Carotid Atherosclerosis Plaques. J. Stroke. 32(3):385
Aulanni’am. 2004. Prinsip dan Teknik Analisis Biomolekul. Fakultas Pertanian Universitas Brawijaya Press, Malng. Indonesia
Bellanti, J.A. 1993. Immunology III. Washington,D.C
Braunwald E. 1997. Heart Disease a textbook of Cardivascular Medicine. 5th Edition. United State, America.
Carranza F.A. 2000. Glikman’s Clinical Periodontology. 8th edition. Philadelpia, London
Gibson, J.R.; H. Chart and R.J. Owen, 1998. Intra-strain Variation in expression of Lipopolysaccharide by Helicobacter pylori. The Society for Applied Microbiology,(26) : 399-403
Hansen P.R. 1995. Roles of Neutrophils in Myocardial Ischemiand Reperfusion. J. Circulation(91):1872-1885
Lee R.T. and P. Libby 1997. The Unstable Atheroma. Atheriosclerosis, Thrombosis and Vascular Biology. (17) :1859-1867
Lee A. and A.P. Moran. 1993. Helicobacter pylori, Basic Mechanisms to Clinical Cure. First edition. Kluwer Academic, Publishers. London. p. 169-177
Merril, C.R., M.E. Pratt. 1986. A silver stain for the rapid quantitative detection of proteins or nucleic acids on membranes or thin layer plates. Anal Biochem
Moran, A.P., I.M. Helander and T.U. Kosunen. 1992. Compositional analysis of Helicobacter pylorirough-form lipopolysaccharides. J Bacteriol(174) : 1370-1377,
Muliarta., Sumarno dan Sulistya, 2005. Deteksi Protein Spesifik Helicobacter pyloriPenderita Infark Miokard Akut Yang Terkait Infeksi Helicobacter pylori. Universitas Brawijaya, Malang
O’Connor S., C. Taylor; L.A. Campbell; Epstein and P. Libby 2001. Potential Infectious Etiologies of Atherosclerosis: A Multifactorial Perspective. CDC Upcoming Issue, (7):5
Opdenakker G, Van den Steen PE & Van Damme J (2001) Gelatinase B: a tuner and amplifier of immune functions. Trends Immunol22(10): 571-579
Romanelli R.; S. Mancini; C. Laschinger; C.M. Overall; J. Sodek and C.A.G. McCulloch 1999. Activation of Neutrophil Collagenase in Periodontitis. Infection and Immunity.69 (5) :2319-2326
Ross R. 1999. Atherosclerosis - An Inflamatory Disease (review). New Eng J. Medicine.(340) : 115 – 12
Sargowo, D. 2006. Update in Unstable Angina: Mechanism of Plug Diruption to Symptoms and the Role of Low Marker Weight Heparin. Fakultas Kedokteran Universitas Brawijaya, Malang.
Shah P.K. and Z.S. Galis 2001. Matrix Metalloproteinase Hypothesis of Plaque Rupture. J. Circulation. (104) : 1878
Sorsa T.; T. Salo; E. Koivunen; Tyynela J, Konttinen YT, Bergmann U, Tuuttila A, Niemi E, Teronen O, Heikkila P, Tschesche H, Leinonen J, Osman S & Stenman UH (1997) Activation of type IV procollagenases by human tumor-associated trypsin-2. J Biol Chem272(34): 21067-21074
Suzanne E.G,et al.2002. Cleavage of intact type I colllagen by MMP-1.Biochemistry.
Tsang K.W. and S.K. Lam 1999. Extragastroduodenal Conditions Associated with Helicobacter pylorInfection. HKMJ(50) :169-174
Visse R & Nagase H (2003) Matrix metalloproteinases and tissue inhibitors of metalloproteinases:structure, function, and biochemistry. Circ Res92(8): 827-839
Weiss SJ (1989) Tissue destruction by neutrophils. N Engl J Med320(6): 365-376
Winarsih, S. 2005. Respons Imun Seluler dan Protektivitas In Vivo Protein Adhesin adh036 Salmonella typhi (Upaya memperoleh Kandidat Vaksin Demam Tifoid). Disertasi, Universitas Brawijaya. Malang
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How to Cite
Sargowo, D., Muliartha, I. K., & Kamaruddin, M. (1). Peran Lipopolisakarida Helicobacter pylori terhadap Aktivitas Neutrofil pada Penderita Infark Miokard Akut melalui Degradasi Kolagen Tipe IV. Indonesian Journal of Cardiology, 28(5), 327-337. https://doi.org/10.30701/ijc.v28i5.218
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Clinical Research
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